Wednesday, August 3, 2011

PLEASE HELP: At physiological pH calculate net charge on Gly-Ala-Gln-Lys-Glu?

I spent 3 hours trying to figure this problem out but still can't get -2 which is the answer. I don't know why my charges don't add up to -2. I'm assuming physiological pH = 7. So amino group of glycine (pka 9.6) would have +1 charge. Lysine R group (pka 10.79) would have +1 charge. And glutamate would have a -2 charge (pka carboxyl is 2.19 and pka R group is 4.25). When i total everything i get a net charge of 0. Can someone tell me what i'm doing wrong? i'd REALLY APPRECIATE any help. thanks.

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